| 质粒名称: | AsCas12a-2C-NLS in pCSDest |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 126637 |
| 存储实验室: | Scot Wolfe |
|
载体骨架基本信息 |
|
| 载体名称: | pCSDest |
| 载体出品公司: | Clontech |
| 空载体大小: | 4086bp |
| 完整质粒大小: | 8163bp |
| 载体类型: | 哺乳细胞表达, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | AsCas12a-2C-NLS、AsCpf1-2C-NLS |
| 物种来源: | Synthetic |
| 插入大小: | 4077bp |
| 启动子: | CMV IE94 promoter |
| 标签/融合蛋白: | 3xHuman influenza hemagglutinin (HA)-Tag (C terminal on insert) |
| 克隆方法: | 未知 |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV |
| 3’测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | AsCas12a-2C-NLS in pCSDest was a gift from Scot Wolfe (Addgene plasmid # 126637 ; http://n2t.net/addgene:126637 ; RRID:Addgene_126637) |
| 参考文献部分: | Enhanced Cas12a editing in mammalian cells and zebrafish. Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184. 10.1093/nar/gkz184 PubMed 30892626 |
买家导航
AsCas12a-2C-NLS in pCSDest载体质粒基本信息
AsCas12a-2C-NLS in pCSDest质粒图谱载体图谱和AsCas12a-2C-NLS in pCSDest载体序列质粒序列多克隆位点信息
AsCas12a-2C-NLS in pCSDest质粒载体简介
AsCas12a-2C-NLS in pCSDest质粒序列载体序列
LOCUS AsCas12a-2C-NLS in pCSDest 8160 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses AsCas12a-2C-NLS in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8160)
AUTHORS Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh
S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J,
Zhu LJ, Lawson ND, Wolfe SA
TITLE Enhanced Cas12a editing in mammalian cells and zebrafish.
JOURNAL Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi:
10.1093/nar/gkz184.
PUBMED 30892626
REFERENCE 2 (bases 1 to 8160)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184."
COMMENT Sequence Label: AsCas12a-2C-NLS in pCSDest
FEATURES Location/Qualifiers
source 1..8160
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 217..235
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 217..234
/label=SP6
/note="SP6 promoter, forward primer"
CDS 282..4202
/codon_start=1
/product="CRISPR-associated protein from Acidaminococcus
sp. BV3L6 (Zetsche et al., 2015)"
/label=AsCpf1
/translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
KDLKLQNGISNQDWLAYIQELRN"
regulatory 3922..3931
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4203..4250
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 4257..4337
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
CDS 4338..4358
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 4394..4528
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4418..4437)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 4472..4491
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(4641..4661)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4641..4659)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4680..4696)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4680..4696)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4693..4715)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4704..4720
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4728..4734,4735..4752,4753..4758))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4773..4794
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4911..4928)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5082..5670)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5162..5181)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5841..6632,6633..6701))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6464..6483
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6702..6806)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6832..7287)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6969..6990)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7181..7200
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter join(7358..8160,1..182)
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
61 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
121 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
181 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
241 agctacttgt tctttttgca ggatcccatc gattcgaatt catgacacag ttcgagggct
301 ttaccaacct gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga
361 ccctgaagca catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact
421 acaaggagct gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc
481 agctggtgca gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga
541 aaaccgagga gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc
601 acgactactt catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg
661 agatctacaa gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg
721 gcaccgtgac cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa
781 cctacttctc cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca
841 cagccatccc acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca
901 tcttcacacg cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga
961 aggccatcgg catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata
1021 accagctgct gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc
1081 gggaggcagg caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga
1141 agaatgatga gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta
1201 agcagatcct gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg
1261 aggaagtgat ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg
1321 agacagccga ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca
1381 gccacaagaa gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga
1441 atgccctgta tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg
1501 agaaggtgca gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg
1561 caggcaagga gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac
1621 acgccgccct ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc
1681 tgaagtctca gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg
1741 atgagtccaa cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga
1801 tggagccttc tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact
1861 ccgtggagaa gttcaagctg aactttcaga tgcctacact ggcctctggc tgggacgtga
1921 ataaggagaa gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca
1981 tcatgccaaa gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca
2041 gcgagggctt tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa
2101 agtgcagcac ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc
2161 tgctgtccaa caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca
2221 atcctgagaa ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga
2281 agggctacag agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt
2341 ataccaagac aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc
2401 tgggcgagta ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg
2461 ccgagaagga gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata
2521 acaaggactt tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg
2581 gcctgttttc tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc
2641 tgttctaccg ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc
2701 tgaacaagaa gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt
2761 acgactatgt gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc
2821 ccaacgtgat caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg
2881 acaagttctt tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta
2941 agttcaacca gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca
3001 tcgatcgggg cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc
3061 tggagcagcg gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca
3121 gggagaagga gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc
3181 tgaagcaggg ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc
3241 aggccgtggt ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg
3301 ccgagaaggc cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg
3361 tgctgaagga ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag
3421 accagttcac ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg
3481 ccccatatac atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa
3541 ccatcaagaa tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg
3601 acgtgaaaac cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga
3661 ggggcctgcc cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt
3721 ttgacgccaa gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc
3781 acagattcac cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg
3841 aggagaaggg catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg
3901 acgattctca cgccatcgac accatggtgg ccctgatccg cagcgtgctg cagatgcgga
3961 actccaatgc cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg
4021 tgtgcttcga ctcccggttt cagaacccag agtggcccat ggacgccgat gccaatggcg
4081 cctaccacat cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc
4141 tgaagctgca gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca
4201 acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc
4261 catacgatgt tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg
4321 atgtccccga ctatgcccca aagaagaagc ggaaggtcta atgatctaga actatagtga
4381 gtcgtattac gtagatccag acatgataag atacattgat gagtttggac aaaccacaac
4441 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
4501 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
4561 ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc
4621 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
4681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
4741 gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
4801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
4861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
4921 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4981 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
5821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
5881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
5941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
6181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
6241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
6301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
6361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
6421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
6481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
6541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
6601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
6841 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
6901 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
6961 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
7021 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
7081 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
7141 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
7201 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
7261 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
7321 aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gaccgccaat tcaatatggc
7381 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
7441 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
7501 ggacttggca ccatgccaat tcaatatggc ggacctggca ctgtgccaac tggggagggg
7561 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
7621 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
7681 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
7741 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
7801 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
7861 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
7921 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
7981 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
8041 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
8101 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aagtacgcca
//
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AsCas12a-2C-NLS in pCSDest质粒载体应用举例
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